Fluoroscence/Fluorescence Microscopy

Equipment: Nikon TE-2000

Working Principle

  • Incident light interacts with a sample
  • Sample reemits light that is then picked up by the photodetector
  • Emission of light during illumination is fluorescence
  • Continued emission of light after illumination is phosphorescence

Technical Information

  • Thin film material characterization
  • Identification of cell components
  • Quality control tool to detect contaminants in chemistry and cosmetics
  • Nanoparticle and quantum well dimensions
  • Characterization of fluorescence and phosphorescence energy states

Imaging Colonies of Human Embryonic Kidney Cells